WebFigure 1. (A) Experimental outline for comparative kinome profiling of undifferentiated (monocytic) and differentiated (macrophage-like) THP-1 cells. Cells were metabolically labeled (SILAC) and differentiation was induced by addition of phorbol-12-myristate 13-acetate (PMA) for 3 days followed by a period of resting for 1 day in PMA-free medium. WebOct 17, 2024 · THP-1 cells were incubated with 50 μg/mL PtNPs for 24 h, then washed twice and resuspended in PMA for the differentiation. After the recovery time, IL-1β, IL-6, IL-8, MCP-1, MIP1β, RANTES and TNF-α release by differentiated THP-1 cells were quantified using the Bio-Plex MAGPIX Multiplex Reader (Bio-Rad, Hercules, CA, USA) according to …

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Web(E) THP-1 cells were incubated with LPS (100 ng/ml) for 0–72 h before measuring macrophage differentiation in comparison to a positive control of cells treated with 10 nM PMA for 72 h. Representative images of THP-1 cell morphology on inversion light microscope at 40x magnifications. WebSep 26, 2024 · The THP-1 cells were incubated with 100 ng/ml PMA for 0, 1, 2 and 3 days respectively, and unattached cells in the supernatant were counted. The cell adherence rate was calculated according to the cell number of THP-1 cells without stimulation. Flow cytometry. The THP-1 cells were incubated with 100 ng/ml PMA for 0, 1, 2 and 3 days … recepty ryby

How the THP-1 cells become adherent with PMA ( what is …

WebJan 15, 2014 · Previously we have published a protocol for transfection of THP-1 macrophages, which in brief pre-differentiates THP-1 monocytes for 48h using 100ng/ml … WebAug 7, 2008 · 160 nM PMA (Sigma) in RPMI medium was added to differentiate the THP-1 cells for 72 h at 37°C for all the cytokine assays through this study. The criteria for the differentiation of the THP-1 cells, adherence, morphological changes, and changes in expression of the cell surface makers such as integrin, FcγRI, CD4, and MHC class II … WebFor Western blot analysis, 1.6 x 10 6 THP-1 cells were PMA-differentiated into macrophages in 6-well plates (Thermo Scientific). On the next day, cells were incubated with or without the indicated inhibitors for 90 min in RPMI supplemented with 1% FCS (Sigma-Aldrich) ... recepty snrl