Cish antibody facs
WebCISH Proteins (Cytokine Inducible SH2-Containing Protein (CISH)) The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. WebMany biological assays use antibodies to detect CISH, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or …
Cish antibody facs
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WebCISH/CIS-1 Antibody Summary. Immunogen. Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CIS. The exact sequence is proprietary. Predicted Species. … WebCISH has been also shown to be a critical immune checkpoint in NK cells 12 and we recently contributed to show the importance of CISH in NK cell homeostasis using a Cish KO germline mouse model. 13 However, we still do not fully appreciate mechanistically how CISH regulates NK antitumor functions.
WebAnti-CISH/CIS antibody - C-terminal (ab191447) Datasheet. SDS. Submit a review Submit a question. $535 Product size. 100 µg. Add to basket. The lead time on this item is … WebAll lanes : Anti-CISH/CIS antibody (ab88383) at 1 µg/ml Lane 1 : CISH/CIS transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Predicted band size: 29 kDa Observed band size: 35 kDa why is the actual band size different from the predicted?
WebAll lanes : Anti-CISH/CIS antibody - C-terminal (ab191447) at 0.5 µg/ml Lane 1 : Rat liver tissue lysate Lane 2 : Rat kidney tissue lysate Lane 3 : Human placenta tissue lysate Lane 4 : A431 cell lysate Lane 5 : SMMC cell lysate Lane 6 : HeLa cell lysate Lane 7 : COLO320 cell lysate Lane 8 : M231 cell lysate Predicted band size: 29 kDa Observed band size: 29 … WebCIS contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling.
WebIn situ hybridization (ISH) is a powerful technique for localizing specific nucleic acid targets within fixed tissues and cells, allowing you to obtain temporal and spatial information …
WebDec 5, 2024 · We also found that Cish−/− GM-MØs had lower cell-surface expression of CD115 (Macrophage-Colony Stimulating Factor receptor; M-CSFR) than WT GM-MØs, while GM-MØs of both genotypes expressed... list shakespeare\\u0027s playsWebFlow Cytometry Antibodies; Isotype Controls; Proteins and Peptides. Recombinant Proteins; Native Proteins; Enzymes; Enzyme Inhibitors; Growth Factors; Peptides; ... using CISH Antibody(A1-1587). A1-1587 was diluted at 1:500 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per … impact fertiliser blendsWebAntibody reactive against mammalian transfected lysate. Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. FACS analysis of negative control 293 cells (Black) and GPRC5D expressing 293 cells (Green) using GPRC5D purified MaxPab mouse polyclonal antibody. Western Blot analysis of GPRC5D expression in transfected 293T … impact fertiliser mackayCISH/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO (4). The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown (4). CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity (4-6). impact fertilisersWebThis CISH antibody is un-conjugated Application All applications for CISH antibodies Immunofluorescence (IF) Characteristics Polyclonal Antibody Purification Affinity purification Immunogen Recombinant fusion protein of human CISH (NP_659508.1). Isotype IgG Alternatives (show) Application Details (hide) Application Notes IF 1:50-1:200 … list share permissions powershellWebCISH is a highly sensitive and reliable method for detecting macrolide-resistant H. pylori in formalin-fixed, paraffin-embedded biopsy specimens, which represents the routine … impact fertilisers hobartWebJul 21, 2024 · ILC2s can be activated by multiple cytokines, including TSLP, IL-25, and IL-33, which have been shown to coordinately regulate the basal output of ILC2s in vivo. 20 However, Cish expression in... listshack reviews